Gastrotheca albolineata (common name: white-lined treefrog) is a frog species in family Hemiphractidae. It is endemic to southeastern Brazil and known from São Paulo, Rio de Janeiro, and Espírito Santo states at elevations of 600–1,400 m (2,000–4,600 ft) asl. Gastrotheca albolineata is a canopy dweller in primary and old secondary rainforests. It requires big trees as its habitat. Females carry the eggs on their back, and eggs develop directly to froglets.