The trispot darter (Etheostoma trisella) is a species of ray-finned fish in the perch family. It is endemic to northern Georgia and southern Tennessee in the United States, where it occurs in the Conasauga River and its tributaries and historically in the Alabama River system. It requires two interconnecting habitats; outside the breeding season it occupies the peripheral zones of the main river, with slow-moving water and silt gravel substrates with vegetation cover; during the breeding season it moves to warmer water with a clay-bottomed substrate with much vegetation. The population of this fish is declining due to loss of suitable habitat because of stream impoundment and land development. The International Union for Conservation of Nature has assessed its conservation status as being