Chilelopsis serena is a mygalomorph spider of Chile, named after its type locality: La Serena, Elqui, Region IV. Males differ from those both C. calderoni and C. puertoviejo in the absence of a patch of setae on the apical tibia I, and also in the palpal tibia having dorsal spines and the bulbal duct being abruptly narrowed at the apex. Females differ from the two other species by their divided spermathecae.