Pseudopaludicola mystacalis is a species of frog in the Leptodactylidae family.It is found in Argentina, Bolivia, Brazil, Paraguay, and possibly Uruguay.Its natural habitats are moist savanna, subtropical or tropical moist shrubland, subtropical or tropical seasonally wet or flooded lowland grassland, freshwater marshes, intermittent freshwater marshes, pastureland, and seasonally flooded agricultural land.It is threatened by habitat loss.