Gastrotheca antoniiochoai is a rare species of frog in the family Hylidae. It is endemic to Peru and only known from near its type locality near the Wayqecha Biological Station (Cusco Region). It has been collected from cloud forest at elevations of about 2,817–2,845 m (9,242–9,334 ft) above sea level. It appears to be an arboreal species living in bromeliads. The species occurs in the Manú National Park and does not face significant threats.