Myotis escalerai is a European bat in the genus Myotis, found in Spain (including the Balearic Islands), Portugal, and far southern France. Although the species was first named in 1904, it was included in Natterer's bat (Myotis nattereri) until molecular studies, first published in 2006, demonstrated that the two are distinct species. M. escalerai is most closely related to an unnamed species from Morocco. Unlike M. nattereri, which lives in small groups in tree holes, M. escalerai forms large colonies in caves. Females start to aggregate in late spring in maternity colonies, and their young are born in summer. The species spends each winter in hibernation colonies, usually in caves or basements.