Dendropsophus joannae is a species of frog in the family Hylidae. It is known from the Pando Department, northern Bolivia (where its type locality is), western Brazil (Acre and Amazonas states), and Madre de Dios Region of southeastern Peru. It is similar to Dendropsophus leali but is smaller, has a shorter snout, more protuberant eyes, and more tuberculate dorsal skin. The inner iris is red. The species has been found in open habitats with tall grass surrounding small ponds and roadside ditches.