Otospermophilus is a genus of ground squirrels in the family Sciuridae, containing three species. Otospermophilus was formerly placed in the large ground squirrel genus Spermophilus, as a subgenus or species group. Since DNA sequencing of the cytochrome b gene has shown Spermophilus to be paraphyletic to the prairie dogs and marmots, it is now separated, along with six other genera.