Eleutherodactylus glamyrus is a species of frog in the Eleutherodactylidae family. It is endemic to western Cuba and known from isolated populations in Sierra Maestra, in the Granma and Santiago de Cuba provinces. Its natural habitats are closed mesic cloud forests at elevations of 800–1,974 m (2,625–6,476 ft) asl. It is an [arboreal]] species, but eggs are laid on the ground. Although a common in suitable habitat, it is declining in abundance. Major threat to is deforestation caused by agriculture, woodcutting, disturbance from tourist activities, and infrastructure development.